first_imgThe early signs of Creutzfeldt-Jakob disease (CJD)—a rare, incurable brain disorder caused by infectious, misshapen proteins called prions—are difficult to interpret. At first, people may simply feel depressed and can undergo personality changes or bouts of psychosis. By the time memory failure, blindness, and coma set in, typically within a year of infection, death is usually imminent. Now, researchers report that a simple nasal swab may help physicians detect the disease far more accurately and earlier than current methods.Finding simple, noninvasive diagnostic tests is “one of the holy grails” for CJD and other prion diseases, says biochemist Byron Caughey of the National Institute of Allergy and Infectious Diseases’ Rocky Mountain Laboratories in Hamilton, Montana, who helped lead the new work. Although there’s no cure for CJD, early diagnosis is important because it can help rule out other, treatable disorders, and it allows medical personnel to take precautions that prevent the disease from spreading to others through exposure to brain tissue or spinal fluid, he says. A type of the disease called variant CJD in humans results from eating meat infected with mad cow disease.Researchers made a major stride toward better diagnostic methods in 2010, when Caughey and other researchers first described a new technique called the RT-QuIC test. The test requires removing cerebrospinal fluid (CSF) from patients by means of a spinal tap, putting samples into a bath of normally shaped prion proteins, and agitating the solution to encourage any abnormal prion “seeds” in the tissue to latch onto the regular proteins. If even trace amounts of pathogenic protein are present, they rapidly use the normal proteins to create millions of insoluble, fibrous amyloid strands. Researchers believe that these amyloid aggregates, also seen in other neurodegenerative diseases such as Alzheimer’s disease, ultimately cause CJD by interfering with or killing off neurons en masse. After death, the brains of people affected by CJD are so badly damaged that they often resemble Swiss cheese or sponges.Sign up for our daily newsletterGet more great content like this delivered right to you!Country *AfghanistanAland IslandsAlbaniaAlgeriaAndorraAngolaAnguillaAntarcticaAntigua and BarbudaArgentinaArmeniaArubaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBermudaBhutanBolivia, Plurinational State ofBonaire, Sint Eustatius and SabaBosnia and HerzegovinaBotswanaBouvet IslandBrazilBritish Indian Ocean TerritoryBrunei DarussalamBulgariaBurkina FasoBurundiCambodiaCameroonCanadaCape VerdeCayman IslandsCentral African RepublicChadChileChinaChristmas IslandCocos (Keeling) IslandsColombiaComorosCongoCongo, The Democratic Republic of theCook IslandsCosta RicaCote D’IvoireCroatiaCubaCuraçaoCyprusCzech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFalkland Islands (Malvinas)Faroe IslandsFijiFinlandFranceFrench GuianaFrench PolynesiaFrench Southern TerritoriesGabonGambiaGeorgiaGermanyGhanaGibraltarGreeceGreenlandGrenadaGuadeloupeGuatemalaGuernseyGuineaGuinea-BissauGuyanaHaitiHeard Island and Mcdonald IslandsHoly See (Vatican City State)HondurasHong KongHungaryIcelandIndiaIndonesiaIran, Islamic Republic ofIraqIrelandIsle of ManIsraelItalyJamaicaJapanJerseyJordanKazakhstanKenyaKiribatiKorea, Democratic People’s Republic ofKorea, Republic ofKuwaitKyrgyzstanLao People’s Democratic RepublicLatviaLebanonLesothoLiberiaLibyan Arab JamahiriyaLiechtensteinLithuaniaLuxembourgMacaoMacedonia, The Former Yugoslav Republic ofMadagascarMalawiMalaysiaMaldivesMaliMaltaMartiniqueMauritaniaMauritiusMayotteMexicoMoldova, Republic ofMonacoMongoliaMontenegroMontserratMoroccoMozambiqueMyanmarNamibiaNauruNepalNetherlandsNew CaledoniaNew ZealandNicaraguaNigerNigeriaNiueNorfolk IslandNorwayOmanPakistanPalestinianPanamaPapua New GuineaParaguayPeruPhilippinesPitcairnPolandPortugalQatarReunionRomaniaRussian FederationRWANDASaint Barthélemy Saint Helena, Ascension and Tristan da CunhaSaint Kitts and NevisSaint LuciaSaint Martin (French part)Saint Pierre and MiquelonSaint Vincent and the GrenadinesSamoaSan MarinoSao Tome and PrincipeSaudi ArabiaSenegalSerbiaSeychellesSierra LeoneSingaporeSint Maarten (Dutch part)SlovakiaSloveniaSolomon IslandsSomaliaSouth AfricaSouth Georgia and the South Sandwich IslandsSouth SudanSpainSri LankaSudanSurinameSvalbard and Jan MayenSwazilandSwedenSwitzerlandSyrian Arab RepublicTaiwanTajikistanTanzania, United Republic ofThailandTimor-LesteTogoTokelauTongaTrinidad and TobagoTunisiaTurkeyTurkmenistanTurks and Caicos IslandsTuvaluUgandaUkraineUnited Arab EmiratesUnited KingdomUnited StatesUruguayUzbekistanVanuatuVenezuela, Bolivarian Republic ofVietnamVirgin Islands, BritishWallis and FutunaWestern SaharaYemenZambiaZimbabweI also wish to receive emails from AAAS/Science and Science advertisers, including information on products, services and special offers which may include but are not limited to news, careers information & upcoming events.Required fields are included by an asterisk(*)The RT-QuIC test, now being used in diagnostic centers around the world, works well but still misses about 10% to 20% of cases, Caughey says. It also requires an invasive, potentially painful spinal tap. In the new study, the researchers ran a fiber optic scope straight up patients’ noses to the very top of the nasal cavity where olfactory neurons are located. In theory, these neurons should contain much higher levels of abnormal prion protein tangles than does CSF because they are directly connected to the brain, Caughey notes. Then, the scientists rolled a small brush along the roof of the nasal vault and analyzed the samples of discharge or tissue they collected with the RT-QuIC assay.As predicted, the concentration of abnormal prions detected in the nasal samples was several orders of magnitude higher than those found in CSF, the group reports online today in The New England Journal of Medicine (NEJM). Compared with the CSF-based method, which correctly identified only about 77% of positive cases in this study, the swab test caught 97% of 30 CJD-positive patients, missing just one. In addition, the nasal brushings produced no false negative results in 45 disease-free controls, Caughey says.“Much higher numbers of patients and controls need to be tested to work out the actual diagnostic sensitivity” of the test, but it appears to be a promising way of detecting CJD without performing an invasive procedure, Caughey says. One of his colleagues underwent the nasal brush test, he says, and although it was not exactly a pleasant experience, “there’s not much pain involved, apparently.”“This is a big advance,” says Pierluigi Gambetti, a neuropathologist at Case Western Reserve University in Cleveland, Ohio. In principle, the nasal brush test should make it possible to diagnose the disorder much earlier than the CSF–based technique because abnormal prions build up in olfactory neurons more quickly than they migrate out of the brain into spinal fluid, he says.Gambetti, who was not involved in the new study, is co-author on a second NEJM paper appearing online today that shows that an assay similar to the CSF–based RT-QuIC test can pick up very small amounts of abnormal prion protein in the urine of people who have contracted a rare variant of CJD that is transmitted by consuming contaminated meat. A handful of such cases have recently been diagnosed in the United States, he notes, and the urine test could be another quick, affordable way to screen for the disease.Although these new diagnostic methods may help prevent CJD from spreading, they can do little for patients themselves, Caughey says. “Hopefully someday we’ll have a therapy” so that clinicians can intervene before too much damage is done.last_img

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first_imgSAN ANTONIO (AP): San Antonio improved to 26-0 on home court this season by beating Orlando 107-92 on Monday night, led by a season-high 28 points from LaMarcus Aldridge. The Spurs edged within four games of Golden State Warriors in the fight over the top seed in the NBA Western Conference. They also stayed four games ahead of Oklahoma City, who beat Washington, thanks to a triple double from Russell Westbrook. Westbrook had his seventh triple-double of the season as the Thunder won 114-98. Westbrook had 17 points, 13 rebounds and 11 assists, and got the best of his match-up with Washington point guard John Wall. Kevin Durant scored 28 points for the Thunder, who have won 11 of 12. Among other key results, Cleveland were forced to overtime at Indiana, but notched a fifth straight win, 111-106. Atlanta defeated Dallas 112-97 to move into a virtual tie with Miami for top spot in the Southeast Division. San Antonio’s Aldridge shot nine for 13 from the field to pace the Spurs. Australian Patty Mills matched a season-high with 22 points, going three for four on three-pointers. San Antonio found their defensive footing late in the third quarter to separate from Orlando. The Spurs outscored the Magic 24-8 to overcome a 70-69 deficit with 1 1/2 minutes left in the third quarter. Nikola Vucevic led Orlando with 20 points and 10 rebounds. Cleveland’s Kyrie Irving scored 25 points as the Cavaliers won in overtime at Indiana. LeBron James had 24 points – but only seven after half-time – with 12 rebounds, and the Cavaliers extended their lead in the Central Division to nine games and won in Indiana for the first time in six years.last_img

first_imgDAMBULLA, Sri Lanka (CMC): Off-spinner Rahkeem Cornwall made key strikes to limit Sri Lanka A after West Indies A’s batting collapsed, but the hosts held the edge heading into today’s final day of the third and final four-day Test here. Sri Lanka ended day three of the contest at the Rangiri Dambulla International Stadium yesterday on 211 for five in their second innings – already 286 runs ahead. Asela Gunaratne top-scored with 69, Sandun Weerakkody struck 48, while Roshen Silva was unbeaten on 29 at the close. Cornwall, the mainstay of the visitors’ attack during the series, claimed four for 62 to take his tally of wickets for the three-match series to 20. Earlier, West Indies A surrendered their last six wickets for 60 runs to be dismissed for 243 in their first innings and concede a lead of 75 runs. Resuming on 183 for four, they lost their captain Shamarh Brooks for 54 after he had added just 11 runs to his overnight 43, while Cornwall chimed in with 25. Seamer Kasun Madushanka (3-45) and left-arm spinner Lakshan Sandakan (3-54) both claimed three wickets apiece, while pacer Lahiru Gamage ended with two for 50. West Indies A were set back early when Brooks perished in the day’s fifth over with the score on 199, after facing 100 deliveries and striking seven fours. His demise triggered a slide from which the visitors never recovered as the last five wickets went down for the addition of just 32 runs. The only batsman to show resistance was Cornwall, who punched two fours and a six in a busy 30-ball knock. Batting a second time, Sri Lanka were handed a robust start by Weerakkody and Kusal Perera, who put on a brisk 67 off 62 deliveries. Weerakkody faced just 36 balls and struck seven fours, while Perera hit a single four in 31 balls at the crease before he departed to a catch at the wicket off Cornwall. In the bowler’s next over, with five runs added, Weerakkody followed via the leg before wicket, but top-scorer Gunaratne then led a recovery by anchoring two half-century partnerships. The 30-year-old right-hander put on 58 for the third wicket with captain Lahiru Thirimanne, who made 27 off 76 deliveries before adding a further 51 for the fifth wicket with Silva. Gunaratne faced 126 deliveries and struck ten boundaries before falling about 40 minutes before the close to a catch at the wicket off seamer Keon Joseph. ROBUST STARTlast_img

first_img0Shares0000Bayern Munich’s Polish forward Robert Lewandowski, the top-scorer in the Bundesliga last season, reportedly wants to leave to seek a ‘new challenge’. © AFP / Christof STACHEBERLIN, Germany, May 30 – Bayern Munich’s star striker Robert Lewandowski wants to leave the Bundesliga club this summer to seek “a new challenge”, his agent told Sport Bild on Wednesday.“Robert feels that he needs a change and a new challenge in his career,” Pini Zahavi told the weekly. “The managers of Bayern know about it.”Lewandowski, 29, whose Bayern contract runs until 2021, has been linked with Real Madrid while Qatari-backed Paris Saint-Germain and Premier League giants Chelsea and Manchester United are also understood to be interested.“I want to focus on preparing for the World Cup, I’m not thinking about club matters, I have a manager for that,” Lewandowski told reporters in the mountain resort of Arlamow, where the Polish team is preparing for the World Cup.He was the Bundesliga’s top scorer in the just-completed season with 29 goals, the third time he has won the trophy after success in 2015/16, also with Bayern, and 2013/14 while at ex-club Borussia Dortmund.In late February, the Poland international parted ways with his previous agent of 10 years, Cezary Kucharski, and signed up with Zahavi, one of the most powerful agents in world football.Zahavi said that “Robert’s motivations are not money or a specific club, because almost all top clubs would like to sign up the world’s best striker.”He said he hoped Bayern president Uli Hoeness and CEO Karl-Heinz Rummenigge would understand the player’s position.“Everyone in the Bayern club leadership has their own great career to look back on. They should understand his situation,” he told Sport Bild.However, Bayern’s bosses have made it clear that Lewandowski will play for them next season.Rummenigge told Kicker magazine earlier this month that Bayern fans “do not have to worry about Robert Lewandowski and the relationship with Bayern… We have a very good one.”“We know what we have in him and I’m very happy that we still have such a long-term contract,” added Rummenigge.“There’s no need to worry, he’ll play here in Munich again next year.”Magazine Kicker also pointed out that Bayern’s senior bosses have repeatedly informed Lewandowski that there is no possibility of leaving before his contract expires.0Shares0000(Visited 1 times, 1 visits today)last_img

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